Extraction and Separation of Gingerols and Assaying of [6]-gingerol
- VernacularTitle:姜酚的提取分离及[6]-姜酚含量测定
- Author:
Suzhen JIANG
;
Ningsheng WANG
- Publication Type:Journal Article
- Keywords:
Gingerols;
6-gingerol;
Extraction and separation;
Assaying
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To extract and separate crude gingerols and to determine the content of [6]-gingerol in crude gingerols.Methods Crude gingerols were separated from the ginger supercritical-CO2 extracts by silica gel dry column chromatography with solvent system of diethyl ether-n-hexane(7:3).The content of [6]-gingerol in crude gingerols was determined by HPLC.Results [6]-gingerol content in the prepared crude gingerols by silica gel dry column chromatography arrived 52.87 %(m/m).[6]-gingerol had a good linearity in the range of 0.512～ 3.075 ? g,r=0.999 9,and the average recovery was 99.19 %,RSD=1.58 % .Conclusion Silica gel dry column chromatography can be used to quickly,effectively prepare crude gingerols,in which [6]-gingerol content is high,and can supply enough material for further research.The liquid chromatographic analysis of [6]-gingerol is simple,reliable,reproducible and can be used for the quality control of crude gingerols.
