Determination of glaucocalyxin A by HPLC and its protein binding rate in plasma of rat
- VernacularTitle:高效液相色谱法研究蓝萼甲素与大鼠血浆蛋白的结合率
- Author:
Luye CAO
;
Zijun CHEN
;
Yunsen LI
;
Yikui LI
- Publication Type:Journal Article
- Keywords:
glaucocalyxin A;
protein binding rate;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protein binding rate of glaucocalyxin A in plasma of rat. Methods The plasma balance dialysis was used. An HPLC for the quantitative determination of glaucocalyxin A was presented and used to calculate the protein binding rate in plasma of rat. Results There were protein binding rate results of 74.46%, 77.87%, and 75.29% at three various concentrations 20, 10, and 1 ?g/ mL of glaucocalyxin A in plasma of rat. Conclusion The HPLC method used to determine glaucocalyxin A is simple, rapid, and sensitive with good specificity, precision and accuracy, and glaucocalyxin A has medium capacities in protein binding rate in plasma of rat.
