Apoptotic mechanism of leukemic K562 cells induced by mangiferin
- VernacularTitle:芒果苷诱导白血病K562细胞凋亡机制的研究
- Author:
Zhigang PENG
;
Jun LUO
;
Yongrong LAI
;
Shanjun SONG
- Publication Type:Journal Article
- Keywords:
mangiferin;
K562 leukemic cells;
apoptosis
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptosis mechanism of K562 cell lines induced by mangiferin. Methods The mRNA expression levels of apoptosis-related genes including bcl-2, bax, survivin of K562 cells treated by mangiferin (25—200 ?mol/L) for 24, 48, 72, and 96 h were determined by RT-PCR; the BCR/ABL protein P210 level was detected by Western blotting. Results Mangiferin up-regulated bax gene of K562 cells significantly and down-regulated bcl-2 gene slightly, resulting in an enhancement of the ratio of bax/bcl-2. Mangiferin down-regulated the expression levels of P210 in K562 cells in a time-and concentration-dependent manner and so is the expression level of survivin mRNA in K562 cells. ConclusionThe mechanism of mangiferin-induced apoptosis in K562 leukemic cells might be involved in up-regulating the gene expression of bax and down-regulating the mRNA expression of BCR/ABL protein P210, bcl-2, and survivin.
