Human stomach cancer SGC-7901 cell apoptosis induced by n-butanol extract of Lagotis Brevitaba Maxim
- VernacularTitle:短管兔耳草正丁醇提取物诱导胃癌SGC-7901细胞凋亡的研究
- Author:
Yan WANG
;
Yingpeng ZHANG
;
Xingming MA
- Publication Type:Journal Article
- Keywords:
Lagotis Brevituba Maxim;
SGC-7901 cells;
apoptosis
- From:
Chinese Traditional Patent Medicine
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study inhibitive effect of Lagotis Brevituba Maxim extracted on human stomach cancer SGC-7901 cells proliferation and/or induce apoptosis. METHODS: Human stomach cancer SGC-7901 cells were treated with Lagotis Brevituba Maxim extracted from n-butanol of 0.34～21.60 mg/mL for 24 h～72 h.Cell proliferation was evaluated by MTT assay.Morphological changes of apoptosis were examined by fluorescence microscope and electron microscope.DNA fragmentation was visualized by agarose gel electrophoresis.The amount of apoptosis cells was measured by flow cytometry. RESULTS: Growth of SGC-7901 cells was obviously inhibited by Lagotis Brevituba Maxim extract with value from 21.60 mg/mL to 0.34 mg/mL.After incubation of SGC-7901 cells with 2.7 mg/mL Lagotis Brevituba Maxim extract for 48 h, morphological changes of apoptosis were observed.DNA ladder was identified by agarose gel electrophoresis of DNA ladder. CONCLUSION: The n-butanol extract of Lagotis Brevituba Maxim can inhibit SGC-7901 cell growth and induce apoptosis.
