Expression of NF-K B signal fractions in renal cell carcinoma(RCC)tissue and influence of arsenic trioxide(As_2O_3) on NF-K B signal transduction in human RCC 786-0 cell
- VernacularTitle:肾癌组织中NF-kB信号分子的表达及As_2O_3对人肾癌786-O细胞中NF-kB信号通路影响的研究
- Author:
Delin WANG
- Publication Type:Journal Article
- Keywords:
Renal cell carcinoma;
Arsenic trioxide;
786-0 cells;
Nuclear factor kappa B;
Signal transduction
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the correlation of NF-K B signal fractions and RCC onset and development,and influence of As_2O_3 on NF-K B signal transduction in RCC 786-0 cell.Methods:NF-K B DNA binding activity,p65 mRNA,p65,IK- K?/?,I K B?and ICAM-1 protein were investigated in 15 RCC tissues and 10 normal renal tissues,and human RCC 786-0 cell by ICC,ISH,Western blot and EMSA.Results:In RCC tissues,NF-K B DNA binding activity,p65,IKK?/?and I- CAM-1 protein were increased,compared to normal renal tissues(P
