Efects of PLCepsilon siRNA gene transfection on invasive power of human bladder cancer cell lines T24
- VernacularTitle:PLC? siRNA转染对膀胱癌细胞株T24侵袭能力的影响
- Author:
Liping OU
- Publication Type:Journal Article
- Keywords:
PLCepsilon;
siRNA;
Plasmid;
Transfection;
TCCB
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of PLCepsilon siRNA transfection on invasive power of human bladder cancer cell lines T24,and to bring a new method of gene therapy for bladder cancer.Methods:An eukaryotic expression vector containing PLCepsilon siRNA was transfected into T24 and positive cell clones were selected and amplified.Expression of PLCepsilon mRNA was detected by RT-PCR.invasive power of T24 were measured before and after transfection by the membrane invasion culture system(Transwell chamber)and gelatin enzymography(including 72 ku typeIV collagenase and 92 ku typeIVcolagenase analyzed).Results:the result of RT-PCR display that the T24 transfected with pll-PLCepsilon and p12-PLCepsilon vectors had lower expressing of PLCepsilon mRNA than the control groups T24 transfected with HK-A and T24 without transfection.In the membrane invasion culture system the invasion number of T24 transfected with p12-PLCepsilon(26.8?5.8)and the number of T24 transfected with pll-PLCepsilon(25.8?6.2)are both lower than the the control groups T24 transfected with HK-A(33.8?5.7)and T24 without transfection(34.8?6.9)(P
