Analysis of Epstein-Barr virus LMP1 gene Xhol-site mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing of China
- VernacularTitle:重庆地区鼻咽癌患者血浆EB病毒LMP1基因检测及N端XhoI酶切位点变异分析
- Author:
Yucheng YANG
- Publication Type:Journal Article
- Keywords:
Epstein-Ban virus;
Latent membrane protein 1;
Nasopharyngeal carcinoma;
Gene mutation
- From:
Journal of Chongqing Medical University
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect and analyze the Epstein-Barr virus LMPI gene XhoI-site mutation in plasma of patients with nasopharyngeal carcinoma(NPC)in Chongqing of China.Methods:DNA extraction and PCR amplification was used in plasma of 48 NPC patients and 40 control non-NPC cases from Chongqing of China.All the PCR production was digested by enzyme XhoI,then segregated in 8% PAGE.XhoI-site mutation was analyzed by sequencing and comparing with B95-8 cell.Results: The LMP1 was amplified and digested successfully from 48 of 48 NPC cases(100%)and from 38 of 40 non-NPC cases (95%),but none of LMPI XhoI-site mutation was found.Sequencing was performed on 4 LMPI genes from NPC cases and 5 LMP1 genes from non-NPC cases,they showed the presence of the XhoI-site.Conclusions:XhoI-site in EBV LMP1 gene N terminus has no mutation in plasma of NPC or non-NPC cases in Chongqing of China.The precise relationship of XhoI-site mutation and NPC pathogenesis need further investigation.
