HPLC Determination of Paeoniflorin in Huachanahuduji and Zhichadupeiji of Meihuadianshe Den
- VernacularTitle:HPLC法测定梅花点舌丹中华蟾酥毒基和脂蟾毒配基的含量
- Author:
Fuli SU
- Publication Type:Journal Article
- Keywords:
Meihuadianshe Dan;
Huachanshuduji;
Zhichadupeiji;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPLC method for determination of Huachanshuduji and Zhichadupeiji in Chansu of Meihuadiansheoan.Metsod HPLC system includes ODS column,4.6mm?250mm,the mobile phase was acetonitrile-water(43.5:56.5),the flow rate was 1.0mL/min,and detection wavelength was 296 nm.The amount of injection was 20?L Results the calibration curves of Huachanshuduji and Zhichadupeiji were linear in the range of 5.31～63.75?g/mL and 6.00～72.00?g/mL(r=0.9998,0.9997). The average recoveries concentration added in samples were 99.45%,99.39%.The deviation was 1.32%, 0.55%(n=6).Conclusion The method is rapid,accurate and reliable.
