Determination of AstragalosideⅣin Ejiao Buxue Koufuye by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定阿胶补血口服液中黄芪甲苷含量
- Author:
Tongxiang LIU
- Publication Type:Journal Article
- Keywords:
HPLC-ELSD;
Ejiao Buxue Koufuye;
AstragalosideⅣ;
determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of AstragalosideⅣin Ejiao Buxue Koufuye.Methods HPLC-ELSD was used to determine directly AstragalosideⅣin Ejiao Buxue Koufuye on Betasil C_(18) column,using CH_3OH-H_2O(74:26)as a mobile phase.The flow rate was 1.0mL/min.The temperature of drift tube for ELSD was 90℃and the flow rate of N_2 was 2.0L/min.Results The linear range of AstragalosideⅣwas 0.43～2.15?g,Y=1.53637X+0.92158,r=0.9999(n=6),the average recovery of the added sample was 98.5% and RSD=0.98%.Conclusion The method is accurate and reliable with a good reproducibility.It can be used for the quality control of Ejiao Buxue Koufuye.
