Studies on Anti-inflammatory Mechanism of Sinomenine
- VernacularTitle:青藤碱抗炎作用机理研究
- Author:
Ruiyan LIANG
;
Liuying CAO
;
Wenjun WANG
;
Xinying LIU
;
Peixun WANG
- Publication Type:Journal Article
- Keywords:
SINOMENINE /pharmacology;
INFLAMMATION/TCD therapy;
GENE EXPRESSION REGULATION;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】The effect of sinomenine on human cyclo-oxygenase(COX) and interleukin-1?(IL-1?) was observed to explore the anti-inflammatory mechanism of sinomenine.【Methods】Peripheral blood monocytes(PBMC) were isolated and incubated with sinomenine.After incubation,total RNA from PBMC was extracted and the effect of sinomenine on COX and IL-1? expression with or without the stimulation of lipopolysaccharide(LPS) was observed by the method of semi-quantitative reverse transcription polymerase chain reaction(RT-PCR).【Results】Sinomenine had no effect on COX-1 gene expression,had a slight inhibitory effect on COX-2 gene expression and had a strong inhibitory effect on IL-1? gene expression.【Conclusion】 The anti-inflammatory mechanism may be related to the inhibition of inflammatory cytokines.
