Screening of proteins interacting with the Hepatitis B Virus PreS1 protein from T7-Phage display library
- VernacularTitle:用T7噬菌体展示技术筛选乙型肝炎病毒PreS1相互作用蛋白
- Author:
Ying HUANG
- Publication Type:Journal Article
- Keywords:
T7-phage display;
Hepatitis B virus PreS1 protein;
Interacting proteins
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen and identify proteins that interact with the hepatitis B virus PreS1 protein by means of T7-phage display system. Method:Hepatitis B virus PreS1 protein was expressed by prokaryotic expression and used as selected molecule to biopan the T7 select human liver cDNA library,the selected positive clones were identified by DNA sequence and analyzed with BLAST program in GenBank. Result:After BLAST in all positive clones,one protein:Cytochrome c Oxidase Subunit I(COX1)was found to interact with the hepatitis B virus PreS1 protein. Conclusion:T7-phage display system is a convenient,rapid and effective method for screening interacting proteins. This results will provide important evidences for studying the pathogenesis and mechanism of HBV.
