The effect of preconditioning with buflomedil hydrochloride on expression of Bcl-2 and Bax on neuron cells of DRG after the sciatic nerve lesion model in rats
- VernacularTitle:盐酸丁咯地尔预处理对大鼠坐骨神经损害后背根神经元Bcl-2、Bax表达的影响
- Author:
Liping ZHANG
- Publication Type:Journal Article
- Keywords:
Buflomedil hydrochloride;
Preconditioning;
Neuron;
Bcl-2;
Bax;
Rats
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore protective mechanism of buflomedil Hydrochloride on the neuron of damaged Sciatic nerve in rats. Methods:SD rats were divided into normal, model,buflomedil hydrochloride group in random. Preconditioned for 14 days,the sciatic nerve lesion models were performed, the Bcl-2 and Bax positive cells were counted by immmunohistochemistry at the end of 6h,12h,24h,48h,72h,96h,7days respectively. Results:(1) At the end of 12h after the model perfomed,The expressions of Bcl-2 in buflomedil hydrochloride group increased obvionsly than the normal and model groups,peaked at 24~48h,and at the end of 7days,the expressions of Bcl-2 in buflomedil hydrochloride group increased obviously compared with model group.(2)The expressions of Bax in buflomedil hydrochloride groug were lower than the normal and model groups in the same periods,and that was the lowest at the end of 7 days,It was different significantly than that of model group(P
