Influences of arsenic trioxide on the cell cycle of human renal cell carcinoma 786-0 cells in vitro and its mechanisms
- VernacularTitle:As_2O_3对肾癌细胞786-0细胞周期的影响及其机制的研究
- Author:
Delin WANG
- Publication Type:Journal Article
- Keywords:
Arsenic trioxide;
Renal cell carcinoma;
786-0 cells;
Cell cycle;
Apoptosis
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the influence of arsenic trioxide on cell cycle,cyclin and cyclin-dependent kinase inhibitor(CDKI) of human renal cell carcinoma 786-0 cells.Methods:The change of cell cycle was measured by flow cytometric assay(FCM),and its mechanism was analyzed by immunocytochemistry and reverse transcription polymerase chain reaction. Results: Treatment with As2O3 of 2?mol/L or 5?mol/L for 24h,48h and 72h, notably induced a G2/M and G0/G1 cell cycle arrest in 786-0 cells,decreased expression of cyclin A,B1,D1,E(P
