Effects of carbon dioxide on the proliferation and CDK4 and Cyclin D1 expressions of human cervical carcinoma cell line CASKI
- VernacularTitle:CO_2对人宫颈癌细胞株CASKI的增殖及CDK4和Cyclin D1表达的研究
- Author:
Huabin WANG
- Publication Type:Journal Article
- Keywords:
Carbon dioxide;
Cervical carcinoma cell;
Proliferation
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of celioscope carbon dioxide pneumoperitoneum on the growth of cervical carcinoma cell line in vitro. Methods:Cervical carcinoma cells CASKI were cultured in vitro,and then treated with 14mmHg,100% carbon dioxide for 2h. The proliferation,cell cycle and expressions of CDK4 and Cyclin D1 were examined by MTT, FCM and immunocytochemistry respectively. Results:After the treatment of carbon dioxide, the proliferation of cervical carcinoma cells CASKI was significantly increased at the 3th day, the proportion of proliferative phase in the cell cycle was significantly increased, and the expressions of CDK4 and Cyclin D1 were also significantly increased. Conclusion:carbon dioxide may promote the proliferation of cervical carcinoma cell line.
