Improvement for culture of primary neurons of newborn rat cortical tissue
- VernacularTitle:大鼠皮层神经元原代培养方法的改进
- Author:
Huarong YU
- Publication Type:Journal Article
- Keywords:
Cells;
Cultured;
Nervous system;
Rats
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an easier and better primary culture technique suitable to neurons of newborn rat cortical tissure. Methods:Low concentration and shortened time of trypsin digestion and mechanical dissociation were adopted to conduct culture. Results:Under the culture condition in vitro,newborn rat neurons showed similar properties to their counterparts in vivo and typical nerver fiber net was formed. Conclusion:This technique for the primary culture of cortical neurons is an ideal good technique for the general labs.
