Isolation,cultivation,purification and proliferation of different gestational age human embryonic neural stem cells
- VernacularTitle:3～18周胎龄人胚胎神经干细胞的分离、培养及增殖
- Author:
Min FENG
- Publication Type:Journal Article
- Keywords:
Gestation age;
NSC;
Proliferation
- From:
Journal of Chongqing Medical University
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the proliferation regularity of different gestational age human embryonic neural stem cells after them were isolated, cultivated and purified successfully.Methods:3~18 weeks human embryo were obtained under asepsis condition, and divided into 3 groups according to gestation age: A group(3~6w), B group(7~11w), C group(12~18w).The each group of NSCs were isolated and purified after primary culture, and identified by single-cell clone culture and immunocytochemistry.(ICC)To study NSCs growth feature by counting numbers and diameters of neurospheres. Results:The cells could survive and proliferate in vitro which were isolated from 3~6 weeks human embryo pallium and neurocanal or 7~18 weeks human fetus pallium. Identification of the cells showed that they had the typical feature of NSC biology in many aspects,such as growth pattern,morphosis,function feature and surface marker. The numbers and diameters of neurospheres from 3~6w gestation age group are much higher than that from 7~18w gestation age group(P
