Experimental Study of Tongluoningxinfang Decoction Protection on Human Umbailical Vein Endothelial Cells
- VernacularTitle:通络宁心方对人脐静脉内皮细胞保护作用的实验研究
- Author:
Li MA
;
Yingzhi YAO
;
Xiaodong ZHANG
- Publication Type:Journal Article
- Keywords:
Tongluoningxinfang decoction;
human umbilical vein endothelial cells;
experimental research
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protecting effect of Tongluoningxinfang decoction on human umbilical vein endothelial cells. Methods To cultivate endothelial cells and prepare serum with Tongluoningxinfang decoction and Fufangdanshenwan,cells were randomly separated into 8 groups,normal group,model group,treat group (high dose,medium dose,low dose),control group (high dose,medium dose,low dose). Except normal group,add 30% H2O2 to the other groups,and make endothelial cells damaged models. Add serum with drugs to treat groups and control groups,and test NO,ET-1 and P-selection (Ps) respectively. Result NO of model group was significantly lower than normal group,all treat groups and all control groups. ET-1 of model group was significantly higher than all treat groups and all control groups. Ps of model group was significantly higher than normal group,treat groups (smedium dose,low dose) and control groups (high dose,medium dose). It had no significant difference compared with model group,treat group (high dose) and control group (low dose). Conclusion Tongluoningxinfang decoction has the same influence as Fufangdanshenwan on secretion of vein endothelial cells. Tongluoningxinfang decoction and Fufangdanshenwan all can prevent vein endothelial cells from damage induced by H2O2.
