Method Research of Measuring Soluble Protein Contents of Animal Material Rough Extraction Using Coomassie Brilliant Blue
- VernacularTitle:用考马斯亮蓝测定动物药材中可溶性蛋白质含量方法初探
- Author:
Kui QIU
;
Tianrun SI
- Publication Type:Journal Article
- Keywords:
CBB;
Animal material;
soluble protein;
content measuring
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the stability of the Bradford method for determination of soluble protein in animal materials. Method The absorption of CBB G-250 combined with proteins was determined by the UV-Spectrophotometry method with the detection wavelength at 595 nm,and the dried bufo was selected as sample,BSA as control. Results The method showed a good linear relationship between absorption and protein content (r =0.996). The sample was stable with in 10 min (RSD=5.0%). Conclusion This method was reliable,simple and rapid.
