CHANGES IN THE EXPRESSION OF OREXIN AND ITS RECEPTORS IN THE RAT HYPOTHALAMUS DURING PREGNANCY,PARTURITION AND LACTATION
- VernacularTitle:妊娠、分娩以及泌乳期大鼠下丘脑中食欲素及其受体表达的变化
- Author:
Junbo WANG
;
Shucai LING
- Publication Type:Journal Article
- Keywords:
Orexin(hypocretin);
Orexin receptor;
Hypothalamus;
Lateral hypothalamic area;
Competitive RT-PCR;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible role of orexin in reproduction and examine the changes in the expression of orexin and its receptors(OX1R,OX2R) in the rat hypothalamus during pregnancy,parturition and lactation. Methods The expressions of prepro-orexin(prepro-OX),orexin-A,OX1R,and OX2R in the rat hypothalamus during pregnancy,parturition,and lactation were evaluated by competitive reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemistry assay. Results Orexin-A immunoreactive(ir) neurons and the OX1R subtype in neuronal cell bodies were mainly located in the lateral hypothalamic area(LHA) as well as in the magnocellular neurons of the paraventricular and supraoptic nuclei respectively in pregnant and lactating rats.The prepro-OX and OX1R mRNA levels on the 1st day of lactation were significantly higher than that during late pregnancy and lactation.No significant changes of OX2R expression were observed during the various reproductive phases.Conclusion Orexin might be involved in regulating reproductive function in early lactation through their binding sites in hypothalamic PVN and SON.
