EFFECTS OF RNAi-MEDIATED GENE SILENCING OF E-CADHERIN EXPRESSION ON THE BIOLOGIC BEHAVIORS OF HO-8910 CELLS
- VernacularTitle:RNA干扰技术抑制上皮性钙黏附分子表达对HO-8910细胞的影响
- Author:
Jun HU
;
Shujuan SHAO
;
Jinyao ZHAO
;
Jin YANG
;
Peiman YANG
- Publication Type:Journal Article
- Keywords:
HO-8910 cell line;
RNAi;
E-cadherin;
Transwell assay;
Human
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective In many types of epithelial tumors,down-regulation or mutation of the epithelial cell-adherent molecule E-cadherin is associated with an increased invasiveness that can be prevented by the forced expression of the cell-adherent molecule.This suggests that E-cadherin is a latestage tumor suppressor that prevents invasion and metastasis.This study was to investigate cell invasion and migration status of human ovary serous cystadeno carcinoma HO-8910 cell line when the E-cadherin expression was down-regulated with RNA interference(RNAi) technology. Methods E-cadherin siRNA was transfected into HO-8910 cells to inhibit the expression of E-cadherin.The effect of RNAi was detected by immunofluoresence assay and Western blotting.The invasive ability of the cancer cells was determined by Transwell assay. Results After RNAi,the expressions of E-cadherin were significantly decreased from 63.7% to 11.9%(P
