CHANGES OF OREXIN AND ITS RECEPTORS IN THE RAT HYPOTHALAMUS DURING ESTROUS CYCLE
- VernacularTitle:大鼠发情周期下丘脑中食欲素及其受体的变化
- Author:
Junbo WANG
;
Peijun WANG
;
Shucai LING
- Publication Type:Journal Article
- Keywords:
Orexin(hypocretin);
Orexin receptor;
Hypothalamus;
Estrous cycle;
Competitive RT-PCR;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate a possible role of orexin in the regulation of estrous cycle by examining the expression of orexin and orexin receptors(OX1R,OX2R) in the rat hypothalamus during the estrous cycle. Methods The levels of prepro-orexin(prepro-OX),orexin-A,OX1R,and OX2R in the rat hypothalamus during the estrous cycle were evaluated by competitive reverse transcription-polymerase china reaction(RT-PCR) assay. Results Only expression OX1R mRNA during late proestrus was significantly higher than that at metestrus.No significant changes of prepro-OX and OX2R mRNA expression were observed during the various estrous cycle phases.Conclusion Orexin might regulate the secretion of GnRH and/or LH by binding OX1R,contributing to the occurrence of ovulation.
