Construction and identification of recombinant plasmid pQE30-hALRIP
- VernacularTitle:重组原核表达载体pQE30-hALRIP的构建及鉴定
- Author:
Jianchuan DENG
- Publication Type:Journal Article
- Keywords:
Human augmenter of liver regeneration;
Prokaryotic expression;
pQE30
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a recombinant prokaryotic expression vector pQE30-hALRIP for further research.Method:PCR was used to amplify hALRIP codon region.The gene was inserted into pMD18-T vector and sequenced.Then the prokaryotic expression vector pQE30-hALRIP was constructed and identified. Results:Clonal recombinant vector pMD18- hALRIP and expression vector pQE30-hALRIP were constructed. DNA sequence analysis and restriction analysis showed both of the vectors were the same as the predicted results. Conclusion:Prokaryotic expression vector pQE30-hALRIP is successfully constructed.
