Subcloning,expressing,purifying and primary studing the biological activity of F3T7
- VernacularTitle:重组蛋白F3T7的亚克隆、表达、纯化及生物学活性的初步鉴定
- Author:
Yang LIU
- Publication Type:Journal Article
- Keywords:
Subclone;
Fusion expression;
Anti-angiogenesis
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone the DNA sequence coding the F3T7,generate the fused protein F3T7,and investigate its function. Methods:We constructed prokaryotic expression vector pET32a(+)-F3T7 by subcloning technique,then fusion protein F3T7 was expressed and purified by affinity chromatography.and its activity of inhibiting the perliferation of HUVEC was assayed by MTT, then FITC was adopted to mark F3T7 to test its affinity with HUVEC,and its anti-angiogenses was detected by CAM. Results:We primarily certified that the fusion protein F3T7 selectively bound to HUVEC,inhibited its proliferation,and showed anti-angiogenic activity.Conclusion:F3T7 could have the abilities of binding to HUVEC and anti-angiogenesis
