Determination of the content of ferulic acid in Xiaojin Wan soft capsule by HPLC
- VernacularTitle:HPLC法测定小金丸软胶囊中阿魏酸的含量
- Author:
Xin WANG
- Publication Type:Journal Article
- Keywords:
Xiaojin Wan soft capsule;
Ferulic acid;
High performance liquid chromatography(HPLC)
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for content determination of ferulic acid in Xiaojin Wan soft capsule by high performance liquid chromatography(HPLC).Methods:Analysis was carried out on Shimadzu LC-2010A HPLC.The separation was performed on DiamonsilTM C18 column(4.6?250mm,5?m),with methanol:1% acetic acid(25:75) as mobile phase,the flow rate was 1.0ml/min,column temperature was 25℃ and the detection wavelength was 320nm.Results:A good linear relationship was found within the range of 3~18?g/ml,Regression equation was Y=100708.7X+24(r =0.9991)and the average recovery was 101.1%(RSD=1.86%,n=5).Conclusion:This method was highly sensitive,accurate and well represented,which could be used to determine the content of ferulic acid in Xiaojin Wan soft capsule.
