HPLC Determination of Gardenoside in Xinxue Tablets
- VernacularTitle:HPLC法测定新雪片中栀子苷含量
- Author:
Shudan ZHANG
;
Qiang XU
;
Jiancheng TANG
- Publication Type:Journal Article
- Keywords:
Xinxue Tablets;
Gardenoside;
RP-HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP-HPLC method for the determination of gardenoside in Xinxue Tablets and to provide evidence for ameliorating its quality criterion.Methods HPLC conditions were as follows: C18(4.6?250 mm,5 ?m)column with temperature at 25 ℃,acetonitrile-water(15∶85) as mobile phase with flow rate of 1 mL/min,and the detection wavelength at 238 nm.Results Gardenoside was well separated from other components and had good linearity in the range of 0.128～1.024 ?g(r=0.999 2).The average recovery was 98.67 %and RSD=1.57 %.Conclusion The method is accurate,reproducible,and can be used for the quality control of Xinxue Tablets.
