Simultaneous determination of ligustilide and 6-gingerol in Kuanxuean Soft Capsule by HPLC internal standard method
- VernacularTitle:HPLC内标法同时测定坤血安软胶囊中藁本内酯和6-姜酚的含量
- Author:
Hongmei WEN
;
Guoping PEN
;
Guoyuan ZHU
;
Quan ZHU
- Publication Type:Journal Article
- Keywords:
Kuanxuean Soft Capsule;
HPLC internal standard method;
ligustilide;
6-gingerol
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a convenient liquid chromatography-internal standard method for determining ligustilide and 6-gingerol in Kuanxuean Soft Capsule (Radix Angelicae Sinensis, Rhizoma Zingiberis Preparatum, etc.) METHODS: Ligustilide, 6-gingerol and the internal standard naphthalene were separated on a Kromasil C_ 18 column. The mobile phase consisted of methanol-trifluroacetic acid (67 ∶ 33, V/V) and was determined at 218 nm. The content of ligustilide and 6-gingerol was calculated by the internal standard method. RESULTS: The recovery of ligustilide was 98.64% (RSD=1.58%); the recovery of 6-gingerol was 97.25% (RSD=1.40%). Result of the standard curve method accords with that of internal standard method. CONCLUSION: The method is sensitive, stable, practical, and suitable for the quality control of Kuanxuean Soft Capsule.
