Determination of Chlorogenic Acid in Jinyin Granules by HPLC
- VernacularTitle:HPLC法测定金茵颗粒剂中绿原酸的含量
- Author:
Tao LI
;
Lin QI
;
Shu MIAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Jinyin Granules;
chlorogenic acid;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method to determind the content of chlorogenic acid in Jinyin Granules. Method The mobile phase was 0.3%HAc-Methonal(74∶26). The UV detection wavelength was 327 nm. The mobile speed was 0.9 mL/min. Separation column was Kroasil ODS(4.6 mm?250 mm). Result A good linearity was obtained in the range of 65～325 ?g,regression A=24 239 869C-31 292,r=0.999 3. The average recovery was 100.10%,RSD was 0.87%(n=6). Conclusion The method was good for determining chlorogenic acid in Jinyin Granules.
