Adventitious root culture of Salvia miltiorrhiza (Ⅰ)—Effects of various media, saltsintensity, and organic components on adventitious root culture of Salvia miltiorrhiza
- VernacularTitle:丹参不定根组织培养的研究(Ⅰ) 培养基种类、盐强度和有机组分对丹参不定根培养的影响
- Author:
Xiaohong GUO
;
Wenyuan GAO
;
Kefeng LI
- Publication Type:Journal Article
- Keywords:
Salvia miltiorrhiza Bunge;
media;
salt intensity;
tanshinone ⅡA;
protocatechuic aldehyde
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of various media, salt intensity, and organic components on the growth of Salvia miltiorrhiza adventitious roots and the synthesis of tanshinone ⅡA and protoca-techuic aldehyde. MethodsThe adventitious roots were obtained through tissue culture by manipulation of various media, salt intensity, and organic components and the contents of tanshinone ⅡA and protocatechuic aldehyde were determined by HPLC. ResultsThe effect of media MS, LS, B5, White, and SH on adventitious roots of S. miltiorrhiza was observed. Adventitious roots grew better under high salt intensity while secondary metabolite biosynthesis was accelerated under low salt intensity in MS basal medium.The reciprocity of five organic components had significant effect on root growth; glycin favored the synthesis of tanshinone ⅡA; scarcity of one of inosital, glycin, VB1, and VB6 inhibited the synthesis of protocatechuic aldehyde. ConclusionMS Basal medium is used for adventitious root culture. The results show that salt intensity and organic components have significant effects on adventitious root culture of S. miltiorrhiza and secondary melabolite synthesis.
