Establishment of a two-dimensional electrophoresis technique for proteome of HeLa cells
- VernacularTitle:HeLa细胞蛋白质组双向电泳技术的建立
- Author:
Min SONG
- Publication Type:Journal Article
- Keywords:
HeLa cells;
Two-dimensional gel electrophoresis;
Proteome
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish and optimize the two-dimensional gel electrophoresis(2-DE)and the preparation of protein samples for the proteome of HeLa cells.Methods:The proteins of HeLa cell were extracted by three different lysis buffers to do 2-DE.The gel images then were acquired by Amersham ImageMaster VDS-CL to do the comparative analysis with PDQuest(7.4.0)software.Results:The combination of Thiourea and Urea can be favorable for the extraction of proteins,and increase the amount of the detectable spots for the alkaline protein.The application of broad-spectrum protease inhibitor could increase the amount of the detectable protein spots in 2-DE remarkably,and also the alkaline protein spots.Conclusions:A two-dimensional electrophoresis technique for HeLa cells was established and an ideal 2-DE map with higher resolution and better reproducibility for HeLa cells was obtained clearly,which laid a foundation for the further studies on proteome of HeLa cells.
