MicroRNA depresse the expression of MAD2 gene in trophoblastic cells
- VernacularTitle:微小RNA抑制胚胎细胞MAD2基因的表达
- Author:
Qiong SHI
- Publication Type:Journal Article
- Keywords:
MicroRNA;
MAD2 gene;
Quantitative real-time PCR
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discuss the mechanism of down-regulated expression of MAD2 gene in trophoblastic cells by microRNA.Methods:Endogenous MAD2 mRNA level was compared by using quantitative real-time PCR before and after transfected with microRNA plasmid;and the Mad2 protein level was compared by western blot.Results:Ratio of MAD2 mRNA/GAPDH mRNA of control group was 0.1780?0.0688,and after transfected with microRNA recombinant plasmid was 0.1778?0.0689 and 0.1778?0.0670respectively.Statistical study of samples showed they have no instinct different;otherwise the protein level in one of the experimental groups was significantly decreased after transfected with microRNA plamid.Conclusion:This study established microRNA can down-regulate gene expression in mammalian cells at translation level,it is a good foundation to study on the control of gene expression.
