Fingerprints of Fructus Forsythiae by HPLC
- VernacularTitle:连翘的HPLC指纹图谱研究
- Author:
Guoxiang SUN
;
Shanxue MU
;
Zhifei HOU
;
Xiuming YU
- Publication Type:Journal Article
- Keywords:
Fructus Forsythiae;
HPLC;
fingerprint;
index of chromatographic fingerprint;
information index of chromatographic fingerprint
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: A HPLC fingerprint method for the quality control of Fructus Forsythiae has been established. METHODS: The chromatographic fingerprints were determined by injecting 5 ?L of the sample solution each time on a CentriSIL BDS colunm(20 cm?4.6 mm,5 ?m) with the gradient elution solvent system composed of 1% acetate acid water and 1% acetate acid acetonitrile.The flow rate was 1 mL/min,the colunm temperature was maintained at(30?0.15)℃ and the signals were acquired at 265 nm.The chromatographic fingerprints were also evaluated by the chromatographic fingerprint index(F),information index of chromatographic fingerprint(I).(RESULTS:)30 co-possessing peaks were selected as the fingerprint peaks,the similarities among samples of Fructus Forsythiae come from 10 production places and its standard chromatographic fingerprints were calculated taking coffeic acid peak as the reference peak. CONCLUSION: This method with good characteristics and specificity can be used in the quality control of Fructus Forsythiae.
