Oligonucleotide Hybridization Detection Based on Surface Plasmon Resonance Technology
- VernacularTitle:基于表面等离子体共振原理的核酸检测技术
- Author:
Xueyong LIU
;
Yanqiang BAI
;
Chunyan WANG
;
Zhongquan DAI
;
Yinghui LI
- Publication Type:Journal Article
- Keywords:
surface plasmon resonance biosensor;
specificity;
detection limit;
reproducibility;
stability
- From:Space Medicine & Medical Engineering
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a kind of detection technique of nucleic acid based on surface plasmon resonance(SPR) and to set up the foundation of real-time, online space microbial detection. Methods A portable online bio-molecules analyzer based on SPR biosensor was applied. The probe was mercapto-modified at the 5’ end and immobilized on the sensor surface. Then the target sequences in the solution were monitored and sensitivity, specificity and reproducibility of the method were investigated. Results The results showed that detection method with good specificity and sensitivity could realize online detection of target sequence. The system could detect 2.3 nmol/L target sequence, CV value of nine detections was 3.5% and that of thirty detections was 14.7%. Conclusion The established nucleic acid detection method has the advantage of high sensitivity, good specificity and reproducibility, which can be applied in the field of nucleic acid detection.
