Microbial transformation on ginsenoside compound K from total saponins in fruit of Panax ginseng
- VernacularTitle:微生物对人参果总皂苷中人参皂苷化合物K的转化作用
- Author:
Yu CUI
;
Binhui JIANG
;
Ying HAN
;
Yuqing ZHAO
- Publication Type:Journal Article
- Keywords:
the total saponins in the fruit of Panax ginseng C. A. Meyer (SFPG);
ginsenoside compound K (C-K);
strain m14;
microbial transformation
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To apply the microbial transformation to transforming the total saponins in the fruit of Panax ginseng (SFPG) and preparing ginsenoside compound K (C-K). Methods The four microbial strains m14, m3, m8, and m9 were screened and isolated from the soil in the botanic garden planted for P. ginseng and they were used for the microbial transformation of SFPG to optimize the strains. Taking C-K contents as index the microbial transformation was detected and analyzed by TLC and HPLC. Results The strain m14 was found to transform the SFPG efficiently to C-K at first. The optimal culturing and transformation conditions of m14 were obtained: time, 6 d; temperature, 30 ℃; revolution of cradle, 160 r/min; initial pH value, 5.5; substratum concentration, 120 mg/mL. Under the optimal condition, the content of C-K was 41.65 times as much as before transformation by m14. Conclusion The m14 is the most effective strain among the four fungal strains. It is the new way available for the C-K industrialized production.
