Biothermodynamic characteristics of Radix Isatis by microcalorimetry
- VernacularTitle:微量热法研究板蓝根的生物热力学特征
- Author:
Yanling ZHAO
;
Jiabo WANG
;
Xiaohe XIAO
- Publication Type:Journal Article
- Keywords:
Radix Isatis;
microcalorimetry;
Escherichia coli;
biothermokdynamics
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the characteristic biothermokinetic methods in different extract fractions of Radix Isatis using Escherichia coli as model bacteria under biothermodynamics. MethodsMicrocalorimetry was used to obtain the characteristic metabolic growth power-time curves and biothermodynamic parameters of bacteria affected by different extract fractions of Radix Isatis, and evaluate the differences among the different extract fractions of Radix Isatis. Results Different extracting fractions of Radix Isatis could inhibit or promote the growth and metabolism of E. coli in different levels. Water-extract fractions and residues could promote its growth while organic solvent extract fractions did the opposite. Furthermore, with the decline of the solvent polarity, the inhibiting rate of organic solvent extract fraction was decreased and the generation time of bacteria was prolonged. There were steady and distinct differences among the different extract fractions in the characteristic metabolic growth power-time curves, such as the maximum power value's decrease and peak time's delay of water-extract fraction and residue, the specific power enhancement in later stage of organic solvent extract fraction. ConclusionMicrocalorimetry is a new and useful mean for the study on the biothermodynamic parameters and characteristics of Radix Isatis which can be helpful to investigate the other models using microcalorimetry.
