Establishment and optimization of SRAP reaction system in Tinospora sagittata
- VernacularTitle:青牛胆SRAP标记反应体系的建立与优化
- Author:
Bing YANG
;
Tianzhi WANG
;
Yu LUO
;
Lu CHEN
- Publication Type:Journal Article
- Keywords:
Tinospora sagittata (Oliv.) Gagnep;
sequence-related amplified polymorphism (SRAP);
molecular marker
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new method for Tinospora sagittata species identification and genetic map construction. Methods Sequence-related amplified polymorphism (SRAP) was applied for T. sagittata to carry on PCR amplification of its DNA and optimize the reaction parameter grade by grade. Results The stable and reproducible SRAP reaction system of T. sagittata has been developed. Conclusion SRAP is an effective method for T. sagittata identification in molecular degree and it has set up a foundation for the further species identification and genetic map construction of T. sagittata.
