REGULATION OF ASTROCYTIC CONDITIONAL MEDIUM ON THE EXPRESSIONS OF GluR2 AND PICK1 mRNA IN CULTURED HIPPOCAMPAL NEURONS
- VernacularTitle:星形胶质细胞条件培养基对培养海马神经元GluR2和PICK1 mRNA表达的调控
- Author:
Zijian LIU
;
Changgeng ZHU
- Publication Type:Journal Article
- Keywords:
Astrocyte;
Neuron;
AMPA receptor;
RT-PCR;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the regulating mechanism of the astrocytes on the expression of ?-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors(AMPARs) subunit in epileptogenesis. Methods The astrocytic conditional medium(ACM) was collected after being stimulated by glutamate,and then ACM was added to the cultured hippocampal neurons.The expression changes of neuronal GluR2 and protein interacting with C-kinase-1(PICK1) mRNA were detected by RT-PCR. Results In the cultured hippocampal neurons,the GluR2 mRNA expression was significantly decreased at the 2nd,8(th),and 12(th) hours after the administration of ACM compared with that in the control group(P
