INSULIN-LIKE GROWTH FACTOR-1 PROTECTS NEURAL STEM CELLS FROM APOPTOSIS INDUCED BY ?-AMYLOID PEPTIDE_(25-35)
- VernacularTitle:胰岛素样生长因子-1抑制?淀粉样蛋白_(25~35)诱导的神经干细胞凋亡
- Author:
Jingbin CUI
- Publication Type:Journal Article
- Keywords:
Insulin-like growth factor-1;
?-amyloid peptide;
Neural stem cell;
Apoptosis;
Immunocytochemistry;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influences of ?-amyloid peptide(A?) on the proliferation and apoptosis of neural stem cells(NSCs) and to investigate the protective mechanism of insulin-like growth factor-1(IGF-1) from toxicity of A?.Methods NSCs were isolated from E14 SD rats brains and then treated with IGF-1,A? and IGF-1 plus A? respectively.The cells were dyed with trypanblue for the count of dead cells and the death rate.BrdU was used to label cells and to evaluate cell proliferation.NSCs and newborn cells were identified by immunocytochemistry and apoptosis was detected by TUNEL technique.Results Cell death rate was low and there were many BrdU-positive cells,but no TUNEL-positive cells in the medium containing IGF-1.Cell death rate increased strikingly 6-48 hours after A? was added,and a great many TUNEL-positive cells were observed in the group treated with A?.Death rate of cells and TUNEL-positive cells decreased more markedly in the medium containing both IGF-1 and A? than that containing A? only.Conclusion A? promotes death and apoptosis of NSCs,while IGF-1 accelerates the proliferation of NSCs and inhibits NSCs apoptosis induced by A?.
