FUNCTION OF HISTOCHEMICAL TYPE OF SKELETAL MUSCLE FIBER IN RAT AND MYOSIN HEAVY CHAIN
- VernacularTitle:大鼠骨骼肌纤维组织化学分型与肌球蛋白重链的功能
- Author:
Daoli ZHU
- Publication Type:Journal Article
- Keywords:
Muscle fiber;
Muscular subvolume;
Myosin heavy chain isoforms;
Succinate dehydrogenase;
Histochemistry;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To analyse and study the distribution of muscle fiber and the composition of myosin heavy chain isoforms(MHCs) in each muscular subvolume of the lateral gastrocnemius of adult SD rats. Methods Succinate dehydrogenase(SDH) staining was applied to analyse the proportions of muscle fibers of four types and sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE) was also used to measure myosin heavy chain isoforms(MHCs). Results The proportions of types Ⅰ(SO),ⅡX(FO),ⅡA(FOG) and ⅡB(FG) muscle fibers in medial subvolume were(10.2?4.1)%、(2.8?2.1)%、(25.7?9.6)%、(61.3?10.1)% respectively,(15.9?8.2)%、(6.1?2.6)%、(22.8?11.5)%、(55.2?12.6)% in lateral superficial subvolume,(21.3?9.2)%、(9.8?2.5)%、(18.5?8.7)%、(50.4?10.9)% in lateral profound subvolume.The distribution of muscle fibers of four types was of a crisscross and mosaic pattern.Type ⅡX muscle fiber had the smallest transverse area and type ⅡB the biggest,with a medium and a smaller transverse areas for types ⅡA and Ⅰ respectively.They were conformed to MHCs Ⅰ,MHCs Ⅱx,MHCs Ⅱa,MHCs Ⅱb that were separately found in medial subvolume,lateral superficial subvolume and lateral profound subvolume with SDS-PAGE.Conclusion The important function of rat lateral gastrocnemius was flexion of knee joint and extension of ankle joint.The lateral superficial subvolume and the lateral profound subvolume seem to take more part in elevating the calcaneus and small muscular movements,and the medial subvolume plays a role in fast movements when muscular power is needed to a degree greater than 25%.
