Expression of Ngn3 in the donor islets with different purity
- VernacularTitle:神经元素3在不同纯度供体胰岛细胞中的表达
- Author:
Dong XUE
- Publication Type:Journal Article
- Keywords:
Ngn3;
Donor islet;
RT-PCR;
Immunohistochemistry
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of Ngn3 in rats donor islets with different purity,and explore the stem cells in them.Methods:Thirty male adult SD rats were randomly divided into 3 groups.Islets were isolated by digestion method of injecting collagenase into pancreatic duct.Group I was harvested by two digestions without purification;For group Ⅱ(n=10),islets sediments were purified by adding 25% ficoll-400 and for,Group Ⅲ(n=10),by adding (25% and 11%)ficoll-400 in order.Finally we applied immunohistochemisiry and RT-PCR to the location and semi-quantitative analysis to test the expression of Ngn3 in the donor islets with different purity.Results:Islets purity of group I was 43.6%?6.29%,Group II 65.3%?4.40%,and group Ⅲ77.6%?6.36%.The difference of Islet purity was significant in three groups (P
