Study on construction method of diabetic cardiomyopathy models in wistar rats with type 2 diabetes mellitus
- VernacularTitle:2型糖尿病心肌病变模型建立方法的实验研究
- Author:
Changquan HUANG
- Publication Type:Journal Article
- Keywords:
Insulin resistance;
Type 2 diabetes mellitus;
Myocardial cell;
Apoptosis
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the change of myocardial cell and matrix in the rats with type 2 diabetes and to approach the construction method of diabetic cardiomyopathy models in wistar rats with type 2 diabetes mellitus.Method:Sixteen wistar rats of 6 months were randomly divided into normal group(N)and diabetic group(D),with eight rats in each group.Euglycemic insulin clamp technique(EICT)was used to determine insulin resistance(IR).Heart weight(HW)and body weight(BW)of each rat were measured to ratio HW and BW,The apoptosis of myocardial cell was evaluated by TUNEL.,and the ultramicrostructure of.myocardial cell and construction of heart were observed.And immunohistochemistry (SABC method) was used to measure the level of Ⅰand Ⅲ types of collagen protein.Results:compared with group(N),GIR of group(D) was remarkably lower(P
