Effects of Fructus Psoraleae on Proliferation and Differentiation of Cultured Osteoblast Isolated from Neonatal Rat's Calvarium
- VernacularTitle:补骨脂提取物对体外培养新生大鼠颅骨成骨细胞的影响
- Author:
Rongping YANG
;
Qingyao SHOU
;
Yongqin TU
;
Binhao WANG
- Publication Type:Journal Article
- Keywords:
Fructus Psoraleae;
Osteoblast;
Tetrazoline colorimetry
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Fructus Psoraleae on the proliferation and differentiation of cultured osteoblast isolated from neonatal rat's calvarium.Methods Calvarium osteoblasts of new born SD rat were cultured and exposed to different concentrations of Fructus Psoraleae extract,then the content of alkaline phosphatase(ALP)in the cell was measured and the cell proliferation was detected by tetrazoline colorimetry(MTT).Results Ethyl acetate extract and alcohol extract from Fructus Psoraleae can improve the activity of ALP and the proliferation of rat's cultured osteoblasts,the optimum concentration was 2.0?10-3 mg/mL.Conclusion Ethyl acetate extract and alcohol extract from Fructus Psoraleae can stimulate the differentiation and proliferation of osteoblasts in vitro.
