Effect of Serum Containing Jiajian Guishen Pill on Apoptosis of Rat's Granulosa Cells of Ovary
- VernacularTitle:加减归肾丸含药血清对卵巢颗粒细胞凋亡的影响
- Author:
Yun SHI
;
Yuzhen ZHANG
;
Qingfei LIU
- Publication Type:Journal Article
- Keywords:
Granulosa cells;
Apoptosis;
Jiajian Guishen Pill;
Serum containing;
Tonifying kidney and spleen;
Regulating liver and removing blood stasis
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Jiajian Guishen Pill on apoptosis of rat's granulosa cells of ovary.Methods Rats' granulosa cells of ovary were cultured in vitro,stimulated with serum containing Jiajian Guishen Pill.The apoptosis rate was checked by flow cytometer and BCL-2 protein expression by immuno-fluorescence 6 hours later with the serum containing Zishen Yutai Pill and human menopausal gonadotropin(HMG)served as the control drugs.Results The apoptosis rate of the low-dosage group of serum containing Jiajian Guishen Pill was significantly lower than those of the other groups(P
