Preparation of Platycodin-D for Reference Substance
- VernacularTitle:桔梗皂苷D对照品的制备
- Author:
Wei LI
;
Jing ZHANG
;
Huiping LI
;
Zi WANG
;
Yinan ZHENG
- Publication Type:Journal Article
- Keywords:
Platycodin-D;
Separation and preparation;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the preparation techniques for preparing the reference substance of platycodin-D.Methods Raw material of platycodi radix was extracted with 70 %MeOH.The concentrated extract passed through D101 macroporous resin column,then the 40 %alcohol eluate was separated by silica gel column chromatography with chloroform-methanol gradient elution.The fractions contained platycodin-D were collected and purified by HPLC.Results HPLC analysis and normalization of peak areas showed the purity of the platycodin-D was 98.8 %.Conclusions The preparation techniques are simple and high yield,can be used for preparing the reference substance of platycodin-D.
