HPLC Determination of Berberine Hydrochloride in Xiaoyou Capsules
- VernacularTitle:高效液相色谱法测定消幽胶囊中盐酸小檗碱的含量
- Author:
Chunhui ZHANG
;
Zhao YANG
;
Xiuming SHAN
- Publication Type:Journal Article
- Keywords:
Xiaoyou Capsules;
Berberine hydrochloride;
HPLC;
Assay
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the content determination of berberine hydrochloride in Xiaoyou Capsules.Method A PR-HPLC method was adopted with a SHIMADZU RP-ODS C18 column as stationary phase and 0.05 mol/L KH2PO4(adjust pH to 3 with H3PO4)-Acetonitrile(70:30)as mobile phase.The detector wavelength was at 265 nm,column temperature was at 40 ℃and the flow rate was 1.0 mL/min.Results Berberine hydrochloride in Xiaoyou capsules showed a good linearity in the range of 0.010 73～0.214 6 ?g.The average recovery was 99.87 %,RSD was 1.43 %.Conclusion The method is simple,reliable,with a good separation,so it can be used for the quality control of Xiaoyou Capsules.
