Determination of coumarin and flavonoid in Psoralen Corylifolia L. in Compound Buguzhi Sustained-Release Tablet by HPLC
- VernacularTitle:HPLC测定复方补骨脂缓释片中补骨脂香豆素类和黄酮类成分的含量
- Author:
Rongping YANG
;
Binhao WANG
;
Yongqin TU
;
Qingyao SHOU
- Publication Type:Journal Article
- Keywords:
HPLC;
Compound Buguzhi Sustained-Release Tablet;
coumarin;
flavonoid
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine coumarin and flavonoid in Compound Buguzhi Sustained-Release Tablet(Fructus Psoraleae,etc). METHODS: HPLC method was developed.The separation was performed on an ODS column with the mobile phase of CH_3OH-H_2O.The flow rate was 1 mL/min and the detection wavelength was 320 nm. RESULTS: The result showed that there was good linear relation between the area and the concentration of psoralen、isopsoralen、isobavachin、bavachin、corylin、corylifolinin、bvachinin、psoraidin and bavachalcone within 0.4-152.0,26.4-132.0,13.6-66.0,27.2-136.0,31.2-156.0,48.8-244.0,50.4-252.0,36.0-180.0,(8.8)-44.0 ?g/mL.The average recovery of psoralen and bavachin is 98.7% and 97.6%. CONCLUSION: This method is convenient,reliable,and can be used to control the quality of Compound Buguzhi Sustained-Release Tablet.
