Analysis of ginsenosides in Shenfu Injection by HPLC-ELSD
- VernacularTitle:高效液相色谱法—蒸发光散射检测器法测定参附注射液中人参皂苷的含量
- Author:
Li LI
- Publication Type:Journal Article
- Keywords:
Shenfu Injection;
ginsenoside Rg_1、Re and Rb_1;
HPLC-ELSD
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To determine the content of ginsenoside Rg_1、Re and Rb_1 in Shenfu Injection (Radix et Rhizoma Ginseng Rubra,etc.) by HPLC-ELSD. METHODS: Separation was achieved on a Waters ODS column((4.6) mm?150 mm,5 ?m).Elution was carried out using a gradient of acetonitrile and water.Drift tube temperature:120 ℃,gas flow rate:1.8 mL/min. RESULTS: The calibration curves were linear in the range of 0.322-(3.22) ?g for ginsenoside Rg_1,0.290-2.90 ?g for ginsenoside Re,0.508-5.08 ?g for ginsenoside Rb_1. CONCLUSION: The method is simple,accurate,effective and feasible.It can be used to control the quality of Shenfu Injection.
