Extraction and Isolation of the Protein Groups with Anti-tumor Activity from Pinellia Ternata Rhizhome
- VernacularTitle:半夏蛋白抗肿瘤活性组分的提取分离
- Author:
Yun FU
;
Bisheng HUANG
;
Juan LI
- Publication Type:Journal Article
- Keywords:
Pinellia ternata;
Bel-7402;
anti-tumor protein groups;
assay of anti-tumor activity
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate the anti-tumor protein groups from Pinellia ternata rhizome and investigate the anti-tumor activity of these protein groups on Bel-7402 cell. Methods The total raw protein was isolated with sepharose column chromatography. Methyl-thiazolyl-tetrazolinm (MTT) was used to analyze the effect of Pinellia ternata Protein on inhibiting growth of tumor cells. Apoptosis was detected by flow cytometry (FCM). Results The 30% and 60% (NH4)2SO4 deposition part of total proteins from Pinellia ternata rhizome have no certain relationship between quantity and inhibitory action, but protein peak 3 eluted with 0.05 mol/L or 0.1 mol/L NaCl from 30% (NH4)2SO4 deposition part showed a effect of concentration depending. FCM analysis showed that the protein of 30% (NH4)2SO4 deposition part could induce apoptosis. Conclusion The 30% (NH4)2SO4 deposition part of total proteins from Pinellia ternata rhizome could significantly inhibit Bel-7402 growth and induce its apoptosis.
