Determination the Contents of Bruchine and Strychine in Pingxiao Tablet by RP-HPLC
- VernacularTitle:RP-HPLC法测定平消片中马钱子碱和士的宁的含量
- Author:
Ming LI
;
Xian ZHANG
;
Yang PAN
- Publication Type:Journal Article
- Keywords:
Pingxiao Tablet;
Bruchine;
Strychine;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the RP-HPLC method for determination of Bruchine and Strychine in Pingxiao Tablet. Methods The lichrospher C18 (4.6 mm?250 mm, 5 ?m) was Used as the stationary phase. The Mobile phase consisted of water-acetonitrile (60∶40) with 1.7 g sodium dodecylsulphate and 3.4 g KH2PO4 in 1 000 mL mixture. The Detecting wavelength was 254 nm and flow rate was 1.0 mL/min. The column temperature was 25 ℃. Result The linear ranage of Bruchine and Strychine were 0.102～0.918 ?g and 0.208 ～ 1.872 ?g respectively. The recovery rates of bruchine and strychnine were 101.07% (RSD=2.26%) and 99.29% (RSD=2.79%) respectively. Conclusion This method is realiable and accurate, the method can be applied to determination the contents of Bruchine and Strychine in Pingxiao Tablet.
