Content Determination of Icariin in Gushuling Extract by HPLC
- VernacularTitle:高效液相色谱法测定骨疏灵提取物中淫羊藿苷含量
- Author:
Shumei WANG
;
Zhenqiang GAO
;
Chuanping WANG
- Publication Type:Journal Article
- Keywords:
HPLC;
extract;
Icariin;
determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content icariin in the extract of Gushuling. Methods HPLC method was performed on Luna C18 column (250 mm?4.6 mm, 5 ?m). The mobile phase consisted of acetonitrile- water (30∶70) and the UV detective wavelength was set at 270 nm. Results The curve of icariin was linear within 0.416～2.496 ?g, Y=2.0?106X-53 810 (r =0.999 9). Conclusion HPLC method is simple, rapid and accurate, and it is suitable for ananlysis of icariin content in extract of Gushuling .
