Cloning and sequencing of the 16s rRNA intergenic spacer region of proteus mirabilis and acinetobacter iwoffii
- VernacularTitle:奇异变形杆菌、洛菲氏不动杆菌16s rRNA3’-23s rRNA 5’间序列的克隆及测序
- Author:
Lei SHI
- Publication Type:Journal Article
- Keywords:
Proteus mirabilis;
Acinetobacter lwoffii;
16s~23s rRNA intergenic spacer region;
T vector
- From:
Journal of Chongqing Medical University
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone and sequence of 16S~23S r RNA intergenic spacer region of Proteus mirabilis and Acinetobacter lowffii for further identification of these two bacteria by means of molecular probe.Methods: The PCR universal clinical common pathogenic bacteria primers for 16S and 23S rRNA's conserved sequences were designed.Then the genomes of these two bacteria were amplified,T Vector clones were constructed with PMD-18 T Vector ad PCR products.The vectors were sequenced and the sequence was submitted to Genbank.Results: T Vector clones were constructed with products amplified by using universal primers;according to Blast analyses,the results of sequencing were determined as ISR sequences of the two kinds of bacteria.And the reports to Genbank were accepted.Conclusion: Genbank accepted the cloning and sequencing results of Proteus mirabilis and Acinetobacter lwoffii as new sequences.And these sequences can be used to identify the two bacteria by universal primers.
